To familiarize yourself with this interface, read this chapter. You can edit your workspace columns to display the compensation matrix name that is applied to your sample. Once you have a compensation matrix and wish to examine it, you will be using the Matrix Editor. If you acquired your data with single-stain controls and wish to compensate it using FlowJo, read this chapter. CHAPTER 2 – Creating a FlowJo compensation matrix from single stain controls If, for any reason, you want to alter the matrix values, read this chapter. This keyword is read and data in the file are compensated when the data is read into FlowJo. Many data files contain their own pre-defined compensation matrix keyword, which was written when the file was created. CHAPTER 1 – Altering an acquisition matrix Poorly scaled data will effect downstream compensation, so get into the habit of adjusting transforms through the “T” button in FlowJo’s graph window, prior to opening the compensation wizard for single stained control assignment. Note, Compensation should always be preceded by scaling your data appropriately. For your convenience, this document is broken into chapters. In FlowJo you can erase the rough compensation, change it, or make a new compensation matrix with the fcs-files of your single stained cells. CHAPTER 2 Creating a FlowJo compensation matrix from single stain controls back to Compensation Workflow Overview Compensation has been reworked for.
#Flowjo compensation matrix manual#
This page will help acquaint you with the various ways to interact with Compensation Matrices in FlowJo. Maybe a rough manual compensation first is a good ide.
0 kommentar(er)
